voltage clamping meaning in English
电位固定法
电压钳制技术
Examples
- Relationship of the background currents obtained with step and ramp voltage clamp showed a negative phase in outward part of the rectification under 5 . 0mmol lk
而在低钾灌流条件下，该曲线在- 55及- 25mv处分别形成跨越cross over ，即出现一个内向电流区。 - Sheep purkinje fibers were used to investigate early afterdepolarization generation and its possible ionic mechanisms with bielectrode voltage clamp technique . under low k
用双电极电压箝制技术研究了羊心蒲氏纤维早后去极化early afterdepolarization , ead的发生及其离子机制。 - At first , the vrl - cdna plasmids were transformed into tg1 r . coli to enlarge vrl - cdna ; then the plasmids were extracted and cut off with enzymes ; subsequently , vrl - cdnas were transcribed into vrl - inrna by t7 or sp6 polymerase in vitro , following vrl - mrnas were injected into xenopus laevis oocyte to express into vr1 receptor protein ; at the end the vrl + - oocytes were tested by double electode voltage clamp
这项实验中，我们首先将大白鼠vr1 - cdna质粒转入大肠杆菌中进行扩增，然后提取质粒并酶切，然后在体外转录成vr1 - mrna ，接着将vr1 - mrna注射到非洲爪蟾卵母细胞中表达为蛋白质受体，建立了实验模型细胞，最后用双微电极电压钳检测此模型细胞。 - The effect of nociceptin on membrane current of sg neurons in the adult rat spinal dorsal horn under voltage clamp superfusion of nociceptin could induce an outward current on sg neurons of spinal dorsal hom at a holding potential of - 70 mv ; this action was dose - dependent with an ec $ o value of 0 . 23 ^ m and hill coefficient of 1 . 5
Nociceptin在电压钳制下对成年大鼠脊髓背角sg神经元膜电流的影响在钳制电压为刁0mv的情况下，灌流给子nociceptin可以诱致脊髓背角sg神经元产生一外向电流并且呈浓度依赖性，它的ecs 。值为0二3卜m ， hill系数为l 5 。